The Mountain Road has closed, just seven minutes after it was re-opened.
Police say the road is closed between Ramsey Hairpin and the Bungalow while they deal with a two-vehicle crash on the Veranda.
However, the route remains open between the Bungalow and the Creg-ny-Baa.
It comes following the highway being shut between the Creg-ny-Baa and Ramsey today, whilst the one-way system was put in place ahead of tomorrow's first TT practice session.
The one-way system will remain in place throughout the racing fortnight.
Road closed after crash
Worries about fire pollution in Glen Maye
UK businessman wants to hear about mobile signal at sea
Minister quizzed over new Meat Plant director
Yellow weather warning this weekend
Education boss retires
Steam Packet warns of sailing delays today
Know someone who's made a difference to their community?